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phenol red free dmem  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phenol red free dmem
    Phenol Red Free Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenol+red+free+dmem/Phenol+Red/pmc13316585-251-32-35
    Average 99 stars, based on 1 article reviews
    phenol red free dmem - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Mitochondrial calcium uptake drives organelle remodeling to promote inflammasome-dependent cytokine release.
    Article Snippet: .. Following treatment, BMDMs were incubated in 5 μM MitoNeoD and 5 nM MitoTracker Deep Red FM in DMEM (10% FBS and 1% pen–strep) for 20min and the medium was then replaced with phenol red-free DMEM (Gibco). .. All images were acquired with the Andor spinning disk confocal described below, using a 500-ms exposure, 50% laser intensity and excitation/emission 561/620 nm for MitoNeoD and 500ms exposure, 50% laser intensity and excitation/emission 637/700 nm for MitoTracker Deep Red FM.

    Article Title: Arl8b inactivates the Rab11a recycling pathway to promote LAMP1 sorting and lysosome biogenesis
    Article Snippet: THP-1 monocytes were differentiated to macrophages by treatment with 30 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) for 24 h, followed by a resting period of 48 h in PMA-free medium. .. For live-cell imaging and flow cytometry, cells were incubated in phenol red-free DMEM (Gibco) supplemented with FBS as described above. .. All cell lines were routinely tested for Mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza), and cultures were limited to 18 passages.

    Staining:

    Article Title: HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors.
    Article Snippet: .. Cells were preincubated with Ned-19 (1 μM) or vehicle for 1 h followed by gp120 (500 pM) treatment for 4 h. After treatment, cells were stained in phenol red-free DMEM (Invitrogen, Cat. #21063029) with one of the following probes; CellROXTM Deep Red (5 μM, Invitrogen, Cat. #C10422) for ROS (30 min), BODIPYTM 581/591 C11 (10 μM, Invitrogen, Cat. #D3861) for lipid peroxidation (25 min), siRNO (5 μM, SigmaAldrich, Cat. #SCT053) for NO (30 min), RT-AM GSH (1 μM, Kerafast, Inc. MA U.S.A., Cat. #EBY001) for reduced glutathione (10 min), P3 (10 μM, Sigma-Aldrich, Cat. #5.34329) for H2S (20 min), and SSP4 (5 μM, Dojindo, Cat. #SB10-10) for sulfane sulfur (20 min). .. LysoTrackerTM (50 nM) to label endolysosomes and Hoechst 33342 (1 μg/mL, ThermoFisher, Cat. #62249) to label nuclei were added for the final 7 min of incubations.

    Article Title: An EGFR-directed photoimmunotheranostic conjugate enables tumor-specific imaging and potent synergistic antitumor activity.
    Article Snippet: Resistance to anti-epidermal growth factor receptor (EGFR) antibodies represents a major bottleneck in treating EGFR-overexpressing tumors such as skin cancer.. While conventional photodynamic therapy offers a promising alternative, its efficacy is often constrained by poor targeting and limited tissue penetration.. To address this, we developed a photoimmunodiagnostic-therapeutic conjugate (PPC) based on panitumumab and the near-infrared photosensitizer IR808, aiming to integrate tumor-specific targeting, real-time fluorescence imaging, and synergistic therapy.

    Cell Culture:

    Article Title: Epigenetic lockdown of type I interferon sensing and signalling in human pluripotent cells.
    Article Snippet: Passaging as single cells was achieved using Accutase Cell Dissociation Reagent (STEMCELL Technologies) followed by gentle centrifugation and resuspension in media with ROCK Inhibitor (Y-27632, STEMCELL Technologies, 10 μM). .. Cells were cultured on plates treated with 2% GeltrexTM LDEV-Free Basement Membrane Matrix (Thermo Fisher Scientific) in Phenol Red-Free DMEM (Thermo Fisher Scientific) for 1h at 37°C. .. Lentiviral vector production and transduction Lentiviral vectors were produced in 293T by transient co-transfection of the retroviral vector, gag-pol and env encoding constructs with Fugene HD transfection reagent (Thermo Fisher Scientific).

    Article Title: Natural killer cell-derived extracellular vesicles reprogram cellular human immunity to enhance tumor cytotoxicity
    Article Snippet: MDA-MB-231 and MCF7 cell lines were tagged with RFP by lentiviral transduction of the Incucyte Nuclight Red (Sartorius, cat#4625), followed by puromycin (Tocris Bioscience, cat#4089) selection. .. RFP-tagged cells were seeded in a 96-well plate (1 × 10 5 cells/well) and treated with 5 μg/mL mitomycin C (Cayman Chemical, cat#11435) for 1 h at 60%–70% confluence and cultured in phenol red-free DMEM (Gibco, catA1896701). ..

    Membrane:

    Article Title: Epigenetic lockdown of type I interferon sensing and signalling in human pluripotent cells.
    Article Snippet: Passaging as single cells was achieved using Accutase Cell Dissociation Reagent (STEMCELL Technologies) followed by gentle centrifugation and resuspension in media with ROCK Inhibitor (Y-27632, STEMCELL Technologies, 10 μM). .. Cells were cultured on plates treated with 2% GeltrexTM LDEV-Free Basement Membrane Matrix (Thermo Fisher Scientific) in Phenol Red-Free DMEM (Thermo Fisher Scientific) for 1h at 37°C. .. Lentiviral vector production and transduction Lentiviral vectors were produced in 293T by transient co-transfection of the retroviral vector, gag-pol and env encoding constructs with Fugene HD transfection reagent (Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: An EGFR-directed photoimmunotheranostic conjugate enables tumor-specific imaging and potent synergistic antitumor activity.
    Article Snippet: Resistance to anti-epidermal growth factor receptor (EGFR) antibodies represents a major bottleneck in treating EGFR-overexpressing tumors such as skin cancer.. While conventional photodynamic therapy offers a promising alternative, its efficacy is often constrained by poor targeting and limited tissue penetration.. To address this, we developed a photoimmunodiagnostic-therapeutic conjugate (PPC) based on panitumumab and the near-infrared photosensitizer IR808, aiming to integrate tumor-specific targeting, real-time fluorescence imaging, and synergistic therapy.

    Live Cell Imaging:

    Article Title: Arl8b inactivates the Rab11a recycling pathway to promote LAMP1 sorting and lysosome biogenesis
    Article Snippet: THP-1 monocytes were differentiated to macrophages by treatment with 30 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) for 24 h, followed by a resting period of 48 h in PMA-free medium. .. For live-cell imaging and flow cytometry, cells were incubated in phenol red-free DMEM (Gibco) supplemented with FBS as described above. .. All cell lines were routinely tested for Mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza), and cultures were limited to 18 passages.

    Flow Cytometry:

    Article Title: Arl8b inactivates the Rab11a recycling pathway to promote LAMP1 sorting and lysosome biogenesis
    Article Snippet: THP-1 monocytes were differentiated to macrophages by treatment with 30 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) for 24 h, followed by a resting period of 48 h in PMA-free medium. .. For live-cell imaging and flow cytometry, cells were incubated in phenol red-free DMEM (Gibco) supplemented with FBS as described above. .. All cell lines were routinely tested for Mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza), and cultures were limited to 18 passages.



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